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Figure 1: Characterization of DSB-enriched probe. a. Map of the HIS4 recombination hotspot in FX4. An insertion of telomeric DNA with three Rap1 binding sites results in high levels of meiosis-specific DSBs at the position indicated by the arrow (4). The position of the probe (pDN42; 4) used in Fig. 1b is shown by the vertical line adjacent to the map. b. Southern analysis of FX4 meiotic DNA samples used in the microarray experiments. Lanes 1 and 2 contain BglII-treated DSB-enriched DNA (Probe 2) isolated using glass fiber filters (11), and BglII-treated total genomic DNA (Probe 1), respectively. Lane 3 contains HindIII-treated lambda DNA. Arrows indicate the position of the intact 2.9 kb HIS4-BIK1 BglII fragment and the 1.9 kb fragment resulting from the meiosis-specific DSB. |