Unbiased Metagenomic Sequencing for Pediatric Meningitis in Bangladesh Reveals Neuroinvasive Chikungunya Virus Outbreak and Other Unrealized PathogensAmerican Society of Microbiology, 2019
Exploratory proteomic analysis implicates thealternative complement cascade in primary CNSvasculitisNeurology, 2019
Kelch-like Protein 11 Seminoma-associated Paraneoplastic EncephalitisNEJM 2019
Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised ChildrenOxford Academic, 2019
Towards precision quantification of contamination in metagenomic sequencing experimentsMicrobiome, 2019
Metagenomic comparison of tracheal aspirate and mini-bronchial alveolar lavage for assessment of respiratory microbiotaAmerican Journal of Physiology, 2019
Miniaturization and optimization of 384-well compatible RNA sequencing library preparationPLOS One, 2019
vFam is a HMMER3 database of profile hidden Markov models (HMMs) built from all the viral proteins present in RefSeq. Use of the vFam database may provide greater sensitivity for the detection of highly divergent viral proteins than the use of traditional alignment tools, such as BLAST. Click here to go to the vFam download page.
This is an online tool for helping users identify the pre-bake dimensions required to produce features with desired dimensions after baking. The tool will calculate pre-bake dimensions, closing pressures, and look up all empirical measurements of valves within stated tolerances of the specified dimensions.
Fordyce, P.M.*, Diaz-Botia, C.A.*, DeRisi, J.L., & R. Gomez-SjobergSystematic characterization of feature dimensions and closing pressures for microfluidic valves produced via photoresist flow
Submitted to Lab on a Chip (2012)Click here to use the tool.
We are pleased to release PRICE (Paired-Read Iterative Contig Extension), a de novo genome assembler implemented in C++. Its name describes the strategy that it implements for genome assembly: PRICE uses paired-read information to iteratively increase the size of pre-assembled contigs. It was designed to address the challenge of assembling viral genomes that constituted a small minority of the reads within ultra-deep, short-read, metagenomic, shotgun datasets. PRICE has already enabled the discovery of several novel virus genomes from such complex datasets.
The early online edition of the PRICE paper, published in G3, is here.Download PRICE
HMMSplicer discovers splice sites in high throughput sequencing datasets without using gene models. HMMSplicer can also be used to find non-canonical junctions as well. HMMSplicer was benchmarked on publickly available A. thaliana
, H. sapiens
, and P. falciparum
datasets and performs well on all genomes. Information about the datasets tested, including the exact command parameters and the final results, is provided. HMMSplicer is implemented in Python and is freely available for all. Download HMMSplicer! (Updated Nov 25th 2010 - Version: 0.9.5)
VersaCount is a simple application to assist with the counting of cells by microscopy. When used with a numeric keypad, it can significantly increase counting efficiency when compared with a traditional clicker. Although it was designed for malaria work, it can be customized for a wide variety of cell counting applications. VersaCount was written by Charlie Kim.Download VersaCount v1.0
DNA microarrays may be used to identify microbial species present in environmental and clinical samples. However, automated tools for reliable species identification based on observed microarray hybridization patterns are lacking. We present an algorithm, E-Predict, for microarray-based species identification.E-Predict Software Download Page
ArrayOligoSelector (AOS) is an open source program developed by Jingchun Zhu for the purpose of systematically designing gene-specific long oligonucleotide probes for entire genomes. For each open reading frame, the program optimizes oligo selection based upon several parameters, including uniqueness, complexity, secondary structure, GC content, and 3' end proximity.
AOS also is hosted at SourceForge.
This site contains documentation and a user-friendly how-to.
For more information, download an excerpt from Jing's thesis describing the AOS algorithm and implementation here.Download AOS v3.8.4
ArrayMaker 2 provides high performance robotic control of microarrayer robots with an incredibly intuitive, easy to use interface. ArrayMaker 2 is optimized for use with the new generation of ultra fast linear servo driven arrayers, yet it is backwards compatible with the original MGuide style of ball-screw driven arrayers. Download ArrayMaker v2.60