Genome-wide regulatory dynamics of translation in the Plasmodium falciparum asexual blood stages
Florence Caro, Vida Ahyong, Miguel Betegon, Joseph L DeRisieLife, 2014Abstract:
The characterization of the transcriptome and proteome of Plasmodium falciparum has
been a tremendous resource for the understanding of the molecular physiology of this parasite.
However, the translational dynamics that link steady-state mRNA with protein levels are not well
understood. In this study, we bridge this disconnect by measuring genome-wide translation using
ribosome profiling, through five stages of the P. falciparum blood phase developmental cycle. Our
findings show that transcription and translation are tightly coupled, with overt translational control
occurring for less than 10% of the transcriptome. Translationally regulated genes are predominantly
associated with merozoite egress functions. We systematically define mRNA 5′ leader sequences,
and 3′ UTRs, as well as antisense transcripts, along with ribosome occupancy for each, and establish
that accumulation of ribosomes on 5′ leaders is a common transcript feature. This work represents
the highest resolution and broadest portrait of gene expression and translation to date for this
medically important parasite.
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