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We are pleased to release HMMSplicer to the community. This open-source software package discovers splice sites in high throughput sequencing datasets without using gene models. HMMSplicer can also be used to find non-canonical junctions as well. HMMSplicer was benchmarked on publicly available A. thaliana, H. sapiens, and P. falciparum datasets and performs well on all genomes. Information about the datasets tested, including the exact command parameters and the final results, is provided. HMMSplicer is implemented in Python and is freely available for all. HMMSplicer was written by Michelle Dimon. Manuscript is in press at PLoS ONE.Learn more and download HMMSplicer here!