Metagenomic DNA Sequencing for the Diagnosis of Intraocular InfectionsThuy Doan, MD, PhD, Nisha R. Acharya, MD, MS, Benjamin A. Pinsky, MD, PhD, Malaya K. Sahoo, PhD, Eric D. Chow, PhD, Niaz Banaei, MD, Indre Budvytiene, MS, CLS, Vicky Cevallos, MT, Lina Zhong, BS, Zhaoxia Zhou, BS, Thomas M. Lietman, MD, Joseph L. DeRisi, PhD
American Academy of Ophthamology, 2017Abstract: Detection of intraocular infections relies heavily on molecular
diagnostics. A fundamental challenge is that only 100e300 ml of
intraocular fluid can be safely obtained at any given time for
diagnostic testing. The most widely available molecular diagnostic
panel for infections in ophthalmology includes 4 separate pathogendirected
polymerase chain reactions (PCRs): cytomegalovirus
(CMV), herpes simplex virus (HSV), varicella zoster virus (VZV),
and Toxoplasma gondii. Not surprisingly, more than 50% of all
presumed intraocular infections fail to have a pathogen identified.
