Metagenomic Sequencing Detects Respiratory Pathogens in Hematopoietic Cellular Transplant Patients
Langelier C, Zinter MS, Kalantar K, Yanik GA, Christenson S, O'Donovan B, White C, Wilson M, Sapru A, Dvorak CC, Miller S, Chiu CY, DeRisi JL

American Journal of Respiratory and Critical Care Medicine, 2018

Abstract: Our understa nding of transcriptomic responses in acuterespiratory distress syndrome (ARDS) is derived, almostexclusively, from studies that have used samples procured from theperipheral circulation as opposed to the alveolar space (1–4). However,it is not known to what degree genomic signatures captured fromcirculating leukocytes accurately reflect the gene expression patterns ofleukocytes in the lung. We hypothesized that the transcriptional signalsfrom peripheral blood monocytes (PBMs) and alveolar macrophages(AMs) would be distinct in ARDS, and that alterations in thetranscriptional state of immune cells in the lung would providenew insights into the pathogenesis of ARDS. Some of the results of thesestudies have been previously reported in the form of an abstract.